Rapid hypothesis testing in Candida albicans clinical isolates using a cloning-free, modular, and recyclable system for CRISPR-Cas9 mediated mutant and Revertant …

J Liu, AK Vogel, J Miao, JA Carnahan… - Microbiology …, 2022 - Am Soc Microbiol
… efficient homozygous gene deletion and reversion in diverse isolates. … transcriptional
accessibility of the native locus differ significantly from NEUT5 gene expression could be altered. …

[HTML][HTML] Contributions of replicative and translesion DNA polymerases to mutagenic bypass of canonical and atypical UV photoproducts

BN Vandenberg, MF Laughery, C Cordero… - Nature …, 2023 - nature.com
… that yeast DNA polymerase eta (pol η) has varied impact on UV-… transcriptional asymmetry
of mutations in pol η-deficient … revertant to have been observed among the number of yeast

Targeted gene deletions in the dimorphic fungal pathogen Histoplasma using an optimized episomal CRISPR/Cas9 system

CA Rappleye - Msphere, 2023 - Am Soc Microbiol
… of gene targeting crRNAs by Pol(II)-based transcription of a … could facilitate mutation of genes,
we expressed two different … lack the possibility of revertant mutations arising in the coding …

[PDF][PDF] Less is more, natural loss-of-function mutation is a strategy for adaptation

YC Xu, YL Guo - Plant Communications, 2020 - cell.com
… in research on various aspects of LoF mutations, including their … of the gene transcript in
case 1 and affects all transcripts in … revertant mutants could occur in essential gene mutations

… of the fission yeast PHO regulon by Pol2 CTD mutation T4A implicates inositol 1-pyrophosphates as agonists of precocious lncRNA transcription termination

A Garg, S Shuman, B Schwer - Nucleic acids research, 2020 - academic.oup.com
genetics, because it is virtually impossible to obtain a revertant of the rpb1-CTD-T4A allele
that has 27 different … two additional changes (in the dnt1 + and yta12 + genes) that are not …

Functional variability in adhesion and flocculation of yeast megasatellite genes

C Saguez, D Viterbo, S Descorps-Declère… - Genetics, 2022 - academic.oup.com
mutants, but none of them showed an amplification of the FLO1 megasatellite. Using synthetic
FLO1 genes, we found that different … to select flocculation proficient revertants. All strains …

Mitochondrial ribosomal RNA mutations and their nuclear suppressors in yeast

M Bolotin-Fukuhara, F Sor… - … . Proceedings of a …, 2019 - books.google.com
… a mutation which alters simultaneously the transcription of … revertants from various
temperaturesensitive mutations. Since … For example, among the revertants of cs909, we have

Telomerase RNA processing: Implications for human health and disease

N Nagpal, S Agarwal - Stem Cells, 2020 - academic.oup.com
… TERC biogenesis and telomere biology disorders, which have led to a better … Different
impairments caused by TERC mutations have been described, including defects in transcription, …

[HTML][HTML] High-throughput synthetic rescue for exhaustive characterization of suppressor mutations in human genes

F Kobaisi, N Fayyad, E Sulpice, B Badran… - Cellular and Molecular …, 2020 - Springer
mutation exhibit different pathological outcomes or different … Unlike the altered splicing in
pseudo-revertant mutations, the … of fixing mutations directly through genetic engineering, it is …

[HTML][HTML] Effect of Non-Lethal Selection on Spontaneous Revertants of Frameshift Mutations: The Escherichia coli hisF Case

S Del Duca, AM Puglia, V Calderone, M Bazzicalupo… - Microorganisms, 2022 - mdpi.com
… of reverse mutations over time and under different selective … were to test (i) whether the
alteration of this three-dimensional … At the end of the experiments a total of 67 His + colonies were …